Changes between Version 2 and Version 3 of Ticket #8

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Timestamp:
04/25/08 10:36:59 (2 years ago)
Author:
gschadow
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  • Ticket #8 – description

    v2 v3  
    44Each working seed lot should be assayed for infectivity in a sensitive assay in cell cultures. 
    55 
    6  * A plaque assay may be used in Vero or other sensitive cells. Titre should be determined by counting the number of visible plaques 
    7 developed, and results recorded as PFU/ml. 
     6 * A plaque assay may be used in Vero or other sensitive cells. Titre should be determined by counting the number of visible plaques developed, and results recorded as PFU/ml. 
    87 * An immunofocus assay may also be used to measure virus titre. The assay is based on the visualization of infected areas of a cell monolayer by probing with dengue serotype-specific monoclonal antibodies. Results should be recorded as FFU/ml. 
    98 * A tissue culture infectious dose assay may also be used to determine virus titre. Results should be recorded as CCID50/ml.