Changes between Initial Version and Version 1 of Ticket #8

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Timestamp:
04/25/08 10:36:08 (2 years ago)
Author:
gschadow
Comment:

More explanation:

Viral infectivity in a sensitive assay in cell culture using immunofocus technology. The titer is determined by visualizing infected areas of a cell monolayer by probing with virus-specific monoclonal antibodies and results recorded as FFU/ml.

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  • Ticket #8

    • Property priority changed from blocker to critical
    • Property summary changed from FFU?? to Add FFU?

  • Ticket #8 – description

    initial v1  
    22.... 
    33 5. Virus titration for infectivity 
    4 Each working seed lot should be assayed for infectivity in a sensitive 
    5 assay in cell cultures. 
    6 • A plaque assay may be used in Vero or other sensitive cells. Titre 
     4Each working seed lot should be assayed for infectivity in a sensitive assay in cell cultures. 
     5 
     6 * A plaque assay may be used in Vero or other sensitive cells. Titre 
    77should be determined by counting the number of visible plaques 
    88developed, and results recorded as PFU/ml. 
    9 • An immunofocus assay may also be used to measure virus titre. The 
    10 assay is based on the visualization of infected areas of a cell monolayer 
    11 by probing with dengue serotype-specific monoclonal antibodies. 
    12 Results should be recorded as FFU/ml. 
    13 • A tissue culture infectious dose assay may also be used to determine 
    14 virus titre. Results should be recorded as CCID50/ml. 
     9 * An immunofocus assay may also be used to measure virus titre. The 
     10assay is based on the visualization of infected areas of a cell monolayer by probing with dengue serotype-specific monoclonal antibodies. Results should be recorded as FFU/ml. 
     11 * A tissue culture infectious dose assay may also be used to determine virus titre. Results should be recorded as CCID50/ml. 
    1512 
    1613If international reference standards become available, immunizing